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The Animal Ethics Committee of Nantong University approved all animal studies, which were consistent with the Guide for the Care and Use of Laboratory Animals

A senescence -galactosidase assay was conducted using the senescence -galactosidase staining kit (Cell Signaling Technology, 9860) according to the manufacturers instructions, and then stained cells were imaged using a ZEISS Primovert inverted microscope (Carl Zeiss Microscopy)

This atomizer setup was used to deposit samples into the bag for extraction using a vacuum pump and further evaluation using a the TD-GC/MS system

Thioredoxin-linked thiol peroxidase from periplasmic space of Escherichia coli